作为应用离心管柱滤器提取蛋白及亚细胞结构的先驱

英文特生物技术(北京)有限公司成功研发出五十多个全新的生命科学研究领域蛋白提取产品,其中具有多个特色产品,为蛋白提取分 离带来了新方案, 从原有的溶液法转换为柱式提取法。我公司产品 均具有快捷、易用、产量高及重复性好的特性。在短短几年中,产品 以其稳定的品质获得了世界各国越来越多的科研,药物开发及临床诊 断机构的青睐。


Minute ™质膜蛋白和细胞组分分离试剂盒 SM-005

Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit

订购信息: SM-005 / 5870.00 元(50 Preps)
                   SM-005 / 597.00元 (4 Preps)

    描述:

    Minute 膜蛋白提取试剂盒通过离心管柱技术配合特定裂解液可以快速从动物细胞或组织中分离天然的膜蛋白。其原理是细胞/组织通过Buffer A致敏,然后细胞以Z字形路径通过离心管柱。在这个过程中细胞膜会破裂,分离出完整的细胞核,因此最终获得的膜蛋白中不含有核膜和核蛋白污染。通过差速离心和密度离心可以把细胞蛋白分为:总膜,细胞核,细胞质,细胞器及质膜,而无需超高速离心。整个操作过程不超过45分钟即可完成。


    提示:本试剂盒最终获取的为细胞膜囊泡沉淀,如需进行蛋白实验需使用膜蛋白溶解液溶解后进行下游实验,根据不同下游实验推荐使用WA-009WA-010WA-011溶解液


    应用:

    SDS-PAGE、immunoblottings、ELISA、IP、膜蛋白结构分析、MS、2-D、酶活性检测等其他应用。

    特点:

    1、简单,快速,性价比高;

    2、天然细胞组份分离(细胞质, 细胞器和质膜);

    3、真正未变性的膜蛋白–无表面活性剂及EDTA;

    4、100%保留蛋白活性及蛋白间相互作用;

    5、小样品量。

    储存条件:

    储存于-20℃


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