作为应用离心管柱滤器提取蛋白及亚细胞结构的先驱

英文特生物技术(北京)有限公司成功研发出五十多个全新的生命科学研究领域蛋白提取产品,其中具有多个特色产品,为蛋白提取分 离带来了新方案, 从原有的溶液法转换为柱式提取法。我公司产品 均具有快捷、易用、产量高及重复性好的特性。在短短几年中,产品 以其稳定的品质获得了世界各国越来越多的科研,药物开发及临床诊 断机构的青睐。


Minute™ 哺乳动物组织及细胞总脂筏分离试剂盒 LR-039

Minute™ Lipid Raft Isolation Kit for Mammalian Cells/Tissues

订购信息: LR-039 / 6223.00元(20 Preps)

    描述:

    脂筏是富含高水平胆固醇和鞘磷脂的微质膜结构域。脂筏涉及许多细胞过程,包括但不限于信号转导,膜运输和蛋白质分选。脂质修饰的蛋白质和一些跨膜蛋白质集中在脂筏中,而其他蛋白质被排除在外。脂筏还与质膜上的Na+ / K+ ATP酶相关。传统的脂筏分离方法使用蔗糖密度法或OptiPrep密度法,采用超高速离心法分离,需要大量的起始样品,而且该方法繁琐、耗时。为了克服传统方法的缺点,我们利用离心管柱技术开发了这款简单、快速的脂筏分离试剂盒。首先将样品的总膜组分分离,然后用含有非离子表面活性剂的缓冲液处理,最后使用台式离心机离心分离耐表面活性剂组分。整个过程无需使用密度梯度和超高速离心,可以在90分钟内从培养的细胞/组织中高度富集脂筏。


    *如仅需分离质膜脂筏,请使用 Minute™ 质膜脂筏分离试剂盒 Cat#LR-042


    提示:本试剂盒最终获取的脂筏沉淀,如需进行蛋白实验需使用溶解液溶解后进行下游实验,根据不同下游实验推荐使用WA-009WA-010WA-011溶解液

    储存温度:

    4℃


    参考文献:LR-039文献列表
    • 1.Wang, M., Liu, J., Tu, Y., Zhao, Z., Qu, J., Chen, K., ... & Wu, C. (2020). RSU-1 interaction with prohibitin-2 links cell-extracellular matrix detachment to down-regulation of extracellular signal-regulated kinase signalling. Journal of Biological Chemistry, jbc-RA120.

      2.Thorwald, M. A., Cacciottolo, M., Forman, H. J., Morgan, T. E., & Finch, C. E. (2021). Reductions in ApoE and GPx4 highlight the Alzheimer’s disease lipid raft vulnerability. Alzheimer's & Dementia, 17, e054511.https://doi.org/10.1002/alz.054511

      3.Thorwald, M. A., Silva, J., Head, E., & Finch, C. E. (2022). Amyloid futures in the expanding pathology of brain aging and dementia. Alzheimer's & Dementia.

      4.Thorwald, M. A., & Finch, C. E. (2022). Alzheimer brains: increased soluble amyloid peptides, but decreased lipid rafts. Alzheimer's & Dementia, 18, e063802

      5.Edwards-Hicks, J., Apostolova, P., Buescher, J. M., Maib, H., Stanczak, M. A., Corrado, M., ... & Pearce, E. L. (2023). Phosphoinositide acyl chain saturation drives CD8+ effector T cell signaling and function. Nature Immunology, 1-15.

      6.Thorwald, M., Godoy-Lugo, J. A., Silva, J., Head, E., O'Day, P. A., Morgan, T. E., ... & Finch, C. E. (2023). Alzheimer's Disease associations of ferritin and glutathione with oxidative damage and neuronal loss. bioRxiv, 2023-03.

      7.Thorwald, M. A., Godoy‐Lugo, J. A., Forman, H. J., & Finch, C. E. (2023). ApoE4 is associated with higher lipid peroxidation but not protein nitration in AD brains. Alzheimer's & Dementia, 19, e079300.

      8.Lei, Y., Yang, H., Xu, X., Tan, Q., Xiang, Y., Tan, T., ... & Xie, P. (2023). Inducing the Localization of HSP70 to Lipid Rafts by Borna Disease Virus 1 Promotes Its Invasion and Host Cells Apoptosis.

      9.McGill, C. J., Christensen, A., Qian, W., Thorwald, M. A., Lugo, J. G., Namvari, S., ... & Pike, C. J. (2024). Protection against APOE4-associated aging phenotypes with the longevity-promoting intervention 17α-estradiol in male mice. bioRxiv, 2024-03.

      10.Rossi, M., Banskota, N., Shin, C. H., Anerillas, C., Tsitsipatis, D., Yang, J. H., ... & Gorospe, M. (2024). Increased PTCHD4 expression via m6A modification of PTCHD4 mRNA promotes senescent cell survival. Nucleic Acids Research, gkae322.

      11.Chaudhary, N., Kasiewicz, L. N., Newby, A. N., Arral, M. L., Yerneni, S. S., Melamed, J. R., ... & Whitehead, K. A. (2024). Amine headgroups in ionizable lipids drive immune responses to lipid nanoparticles by binding to the receptors TLR4 and CD1d. Nature Biomedical Engineering, 1-16.





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