作为应用离心管柱滤器提取蛋白及亚细胞结构的先驱

英文特生物技术（北京）有限公司成功研发出五十多个全新的生命科学研究领域蛋白提取产品，其中具有多个特色产品，为蛋白提取分 离带来了新方案， 从原有的溶液法转换为柱式提取法。我公司产品 均具有快捷、易用、产量高及重复性好的特性。在短短几年中，产品 以其稳定的品质获得了世界各国越来越多的科研，药物开发及临床诊 断机构的青睐。

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Minute™ 动物细胞/组织内质网富集试剂盒 ER-036

Minute™ ER Enrichment Kit For Tissues and Cultured Cells

订购信息： ER-036 / 5831.00元(20 Preps)

    描述：

    内质网是连接核膜和质膜功能的主要膜结构。内质网在真核细胞蛋白转运的胞外途径中起重要作用。在细胞质中合成的蛋白质被运送到内质网，囊泡将蛋白质运送到高尔基体，然后与质膜融合。传统分离内质网的方法是基于密度梯度超离心，需要大量的起始样品，方法繁琐，耗时，交叉污染严重。目前，所有内质网分离的商用试剂盒都是基于上世纪70年代开发的方法。与市场上的内质网分离试剂盒不同，我们的内质网分离试剂盒使用离心管柱技术，简单、快速，只需少量的起始培养细胞或组织，不需要使用杜恩斯匀浆管和超高速离心，就能从培养的细胞/组织中分离出天然ER(主要是粗面内质网)。整个操作可以在大约2h内完成。

    提示：本试剂盒最终获取的内质网囊泡沉淀，如需进行蛋白实验需使用溶解液溶解后进行下游实验，根据不同下游实验推荐使用WA-009，WA-010，WA-011溶解液。

    储存温度：

    4℃



    产品视频
    参考文献：ER-036文献列表

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      13.Liu, Y. Y., Bai, J. S., Liu, C. C., Zhou, J. F., Chen, J., Cheng, Y., & Zhou, B. (2023). The Small GTPase Rab14 Regulates the Trafficking of Ceramide from Endoplasmic Reticulum to Golgi Apparatus and Facilitates Classical Swine Fever Virus Assembly. Journal of Virology, e00364-23.

      14.Xiao, X., Shi, J., He, C., Bu, X., Sun, Y., Gao, M., ... & Zhang, J. (2023). ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy. Nature Communications, 14(1), 2859.

      15.Li, Y., Li, K., Pan, T., Xie, Q., Cheng, Y., Wu, X., ... & Cui, S. (2023). Translocation of IGF-1R in endoplasmic reticulum enhances SERCA2 activity to trigger Ca2+ ER perturbation in hepatocellular carcinoma. Acta Pharmaceutica Sinica B.

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      18.Wang, W., Tan, J., Liu, X., Guo, W., Li, M., Liu, X., ... & Zhou, Q. (2023). Cytoplasmic Endonuclease G promotes nonalcoholic fatty liver disease via mTORC2-AKT-ACLY and endoplasmic reticulum stress. Nature Communications, 14(1), 6201.

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      21.Bi, Y. Y., Chen, Q., Yang, M. Y., Xing, L., & Jiang, H. L. (2024). Nanoparticles targeting mutant p53 overcome chemoresistance and tumor recurrence in non-small cell lung cancer. Nature Communications, 15(1), 1-18.

      22.Bansal, R., Torres, M., Hunt, M., Wang, N., Chatzopoulou, M., Manchanda, M., ... & Wikstrom, J. D. (2024). Role of the mitochondrial protein cyclophilin D in skin wound healing and collagen. JCI insight.

      23.Chiu, C. C., Chen, Y. L., Weng, Y. H., Liu, S. Y., Li, H. L., Yeh, T. H., & Wang, H. L. (2024). Downregulation of Protease Cathepsin D and Upregulation of Pathologic α-Synuclein Mediate Paucity of DNAJC6-Induced Degeneration of Dopaminergic Neurons. International Journal of Molecular Sciences, 25(12), 6711.

      24.Lee, C., Syed, F., & Evans-Molina, C. miR-146a-5p mediates inflammation-induced β cell mitochondrial dysfunction and apoptosis.bioRxiv

      25.Ryu, G., Ko, M., Lee, S., Park, S. I., Choi, J. W., Lee, J. Y., ... & Kwon, H. J. (2024). Urolithin a Protects Hepatocytes from Palmitic Acid-Induced ER Stress by Regulating Calcium Homeostasis in the MAM.





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