作为应用离心管柱滤器提取蛋白及亚细胞结构的先驱

英文特生物技术(北京)有限公司成功研发出五十多个全新的生命科学研究领域蛋白提取产品,其中具有多个特色产品,为蛋白提取分 离带来了新方案, 从原有的溶液法转换为柱式提取法。我公司产品 均具有快捷、易用、产量高及重复性好的特性。在短短几年中,产品 以其稳定的品质获得了世界各国越来越多的科研,药物开发及临床诊 断机构的青睐。


Minute™ 无表面活性剂的动物细胞/组织总蛋白提取试剂盒 SN-006

Minute™ Detergent-Free Total Protein Extraction Kit for Animal Cells and Tissues

订购信息: SN-006 / 4410.00元 (50 Preps)
                   SN-006 / 459.00元 (4 Preps)

    描述:

    无表面活性剂的动物细胞/组织总蛋白提取试剂盒,由优化细胞裂解液和0.6 ml离心管柱组成,能够迅速从动物细胞(昆虫/哺乳动物等)和组织(无脊椎动物和脊椎动物)中提取无表面活性剂的总蛋白。试剂盒中的裂解液不含任何表面活性剂和EDTA。离心管柱技术提取蛋白质,样品量最小可处理20ul,最大可达500ul。从细胞/组织中提取总蛋白质操作时间小于5分钟,得率可达1-5mg/ml。
    应用:

    提取的蛋白质样品可以应用于蛋白质组学(液相色谱/质谱),IP,ELISA,2D分析,等电聚焦,SDS-PAGE, immunoblottings等。

    特点:

    1、速度快操作简单;

    2、小样本量:最小可处理20ul;

    3、无蛋白丢失,高得率;

    4、无需超声处理, 煮沸, 或反复冻融;

    5、不含任何表面活性剂和EDTA。

    储存条件:

    储存于4℃


    参考文献:SN-006文献列表
    • 1. Seo, J-H. et al (2014). Directing stem cell differenciation by changing the molecular mobility of supramolecular surfaces. Advanced Healthcare Materials. DOI: 10.1002/adhm.201400173
      2. M. Takada, S. Chiba et al. (2015). Inflammatory responses to neutral fatand fatty acids in multiple organs in a rat model of fat embolism syndrome, Forensic Science International . DOI: org/10.1016/j.forsciint.2015.07.011
      3. Seo J. et al. (2016).Dynamic polyrotaxane-coated surface for effective differentiation of mouse induced pluripotent stem cells into cardiomyocytes. RSC Advances. DOI: 10.1039/C6RA03967G
      4. Xu, Wenteng, et al. (2016).Ubiquitin ligase gene neurl3 plays a role in spermatogenesis of half-smooth tongue sole (Cynoglossus semilaevis) by regulating testis protein ubiquitination. Gene (2016), DOI: 10.1016/j.gene.2016.07.062

      5.Yamamoto, H., Hayano, S., Okuno, Y., Onoda, A., Kato, K., Nagai, N., ... & Kato, T. (2020). Phosphorylated proteome analysis of a novel germline ABL1 mutation causing an autosomal dominant syndrome with ventricular septal defect. International Journal of Cardiology.

      6.Pietras, P., Leśniczak-Staszak, M., Kasprzak, A., Andrzejewska, M., Jopek, K., Sowiński, M., ... & Szaflarski, W. (2021). MVP Expression Facilitates Tumor Cell Proliferation and Migration Supporting the Metastasis of Colorectal Cancer Cells. International Journal of Molecular Sciences, 22(22), 12121.

      7.Minerva, D., Othman, N. L., Nakazawa, T., Ito, Y., Yoshida, M., Goto, A., & Suzuki, T. (2022). A New Chemotactic Mechanism Governs Long-Range Angiogenesis Induced by Patching an Arterial Graft into a Vein. International Journal of Molecular Sciences, 23(19), 11208.





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