作为应用离心管柱滤器提取蛋白及亚细胞结构的先驱

英文特生物技术（北京）有限公司成功研发出五十多个全新的生命科学研究领域蛋白提取产品，其中具有多个特色产品，为蛋白提取分 离带来了新方案， 从原有的溶液法转换为柱式提取法。我公司产品 均具有快捷、易用、产量高及重复性好的特性。在短短几年中，产品 以其稳定的品质获得了世界各国越来越多的科研，药物开发及临床诊 断机构的青睐。

我们的产品-蛋白快速提取试剂盒
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Minute™ 皮肤组织总蛋白提取试剂盒 SA-01-SK

Minute™ Total Protein Extraction Kit for Skin Tissue

订购信息： SA-01-SK / 4263.00元(50 Preps)
    描述：

    皮肤组织由表皮、真皮和皮下脂肪组成。由于皮肤组织独特的结构，所以研磨匀浆非常困难，在提取总蛋白时也十分难裂解。使用传统的溶液蛋白质提取方法如RIPA，提取皮肤总蛋白效率，产率都极低。提取的蛋白质图谱也是不完整的。本试剂盒使用离心管柱技术及优化的裂解液，采取物理研磨和化学裂解相结合的方法，为人和动物皮肤组织总蛋白的提取提供了一种高效的方法。本试剂盒可简单快速的提取高质量皮肤总蛋白，无蛋白丢失，可以根据特定的下游应用选择变性细胞裂解液或天然细胞裂解液。整个提取过程不到10分钟即可完成，蛋白质得率在1-5mg/ml。试剂盒所提供试剂及耗材可以满足客户50个样品实验。


    应用：

    SDS-PAGE, WB，IP，ELISA，酶活性测定和蛋白质组学分析。缓冲剂与IMAC树脂兼容，可用于His标签蛋白纯化。在质谱分析之前需将提取的蛋白质样品中的盐和表面活性剂除去。


    储存条件：

    常温


    参考文献：SA-01-SK文献列表

    • 1.Zhang, C., Li, Y., Qin, J., Yu, C., Ma, G., Chen, H., & Xu, X. (2021). TMT-Based Quantitative Proteomic Analysis Reveals the Effect of Bone Marrow Derived Mesenchymal Stem Cell on Hair Follicle Regeneration. Frontiers in Pharmacology, 12.

      2.Wang, T., Zhu, G., Qin, L., Wang, Q., She, C., Xu, D., ... & Li, S. T. (2021). Kininogen–Nitric Oxide Signaling at Nearby Nonexcited Acupoints after Long-Term Stimulation. JID Innovations, 1(3), 100038.

      3.Yin, S., Song, R., Ma, J., Liu, C., Wu, Z., Cao, G., ... & Wang, Y. (2022). Receptor activity‐modifying protein 1 regulates mouse skin fibroblast proliferation via the Gαi3-PKA-CREB-YAP axis. Cell Communication and Signaling, 20(1), 1-18.

      4.Wang, Y., Huang, J., & Jin, H. (2022). Reduction of Methyltransferase-like 3-Mediated RNA N6-Methyladenosine Exacerbates the Development of Psoriasis Vulgaris in Imiquimod-Induced Psoriasis-like Mouse Model. International Journal of Molecular Sciences, 23(20), 12672.

      5.Zhu, W., Dong, Y., Xu, P., Pan, Q., Jia, K., Jin, P., ... & Che, B. (2022). A composite hydrogel containing resveratrol-laden nanoparticles and platelet-derived extracellular vesicles promotes wound healing in diabetic mice. Acta Biomaterialia.

      6.Yuan, X., Tang, B., Chen, Y., Zhou, L., Deng, J., Han, L., ... & Wang, Y. (2023). Celastrol inhibits store operated calcium entry and suppresses psoriasis. Frontiers in Pharmacology, 14.

      7.Zhou, H., Gan, Y., Li, Y., Chen, X., Guo, Y., & Wang, R. (2023). Degradation of Rat Sarcoma Proteins Targeting the Post-Translational Prenyl Modifications via Cascade Azidation/Fluorination and Click Reaction. Journal of Medicinal Chemistry.

      8.Wang, J. Study on the Regulatory Mechanism of Jia Wei Guo Min Decoction On Degranulation of Mast Cell in Ad Mice Based on His/Par-2 Pathway.

      9.Huang, J. L., Xu, Y. H., Yang, X. W., Wang, J., Zhu, Y., & Wu, X. B. (2023). Jiawei guomin decoction regulates the degranulation of mast cells in atopic dermatitis mice via the HIS/PAR-2 pathway. Journal of Ethnopharmacology, 117485.

      10.Wu, Z., Liu, C., Yin, S., Ma, J., Sun, R., Cao, G., ... & Wang, Y. (2024). P75NTR regulates autophagy through the YAP-mTOR pathway to increase the proliferation of interfollicular epidermal cells and promote wound healing in diabetic mice. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 167012.

      11.Xu, X. L., Wu, S. J., Qi, S. Y., Chen, M. M., Liu, Z. M., Zhang, R., ... & Lian, Z. X. (2024). Increasing GSH-Px Activity and Activating Wnt Pathway Promote Fine Wool Growth in FGF5-Edited Sheep. Cells, 13(11), 985.




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