作为应用离心管柱滤器提取蛋白及亚细胞结构的先驱

英文特生物技术(北京)有限公司成功研发出五十多个全新的生命科学研究领域蛋白提取产品,其中具有多个特色产品,为蛋白提取分 离带来了新方案, 从原有的溶液法转换为柱式提取法。我公司产品 均具有快捷、易用、产量高及重复性好的特性。在短短几年中,产品 以其稳定的品质获得了世界各国越来越多的科研,药物开发及临床诊 断机构的青睐。



    订购信息:SN-047 / 4995.00元(20 Preps)

    Minute™ 组织/细胞单个细胞核分离试剂盒 SN-047

    Minute™ Single Nucleus Isolation Kit for Tissues/Cells

    描述:

    从组织和培养细胞中分离和纯化细胞核是常见的实验室操作。离体细胞核,特别是单核,广泛应用于FACS分析、单核分析(如RNA-seq和ATAC-seq)、免疫荧光染色、细胞周期分析和凋亡研究等多种实验。传统的分离细胞核的方法相对简单、直接。然而,经常遇到分离的细胞核聚集和高比例的双核现象等。本试剂盒专门为解决这些问题而设计。该方案简单、快速、有效。与传统方法相比,该试剂盒所需原料少,样品量范围大(1-30mg)。缓冲液中含有专用的表面活性剂混合物,可有效的溶解细胞。如需要不含有表面活性剂的完整细胞核分离,可以选购cat#NI-024(无表面活性剂细胞核更不易聚集)。

    应用:

    分离的细胞核可用于单个细胞核分析(RNA-seq和ATAC-seq),流式细胞仪分析,染色体免疫共沉淀(芯片),免疫荧光染色。

    储存:

    4℃

    *储存或运输细胞核时,推荐使用WA-014抗聚集细胞核存储液存储。

    注意:根据组织类型和有无表面活性剂,我们还提供以下单个细胞核分离试剂盒
    NI-024:Minute™无表面活性剂的单个细胞核分离试剂盒
    BN-020:Minute™神经元组织/细胞单个细胞核分离试剂盒
    AN-029:Minute™脂肪组织/培养脂肪细胞的细胞核和胞浆分离试剂盒


    参考文献:SN-047文献列表
    • 1.Kang, R. B., Li, Y., Rosselot, C., Zhang, T., Siddiq, M., Rajbhandari, P., ... & Lu, G. (2022). Single Nucleus RNA Sequencing of Human Pancreatic Islets In Vitro and In Vivo Identifies New Gene Sets and Three β-Cell Subpopulations with Different Transcriptional Profile. bioRxiv.

      2.de Nicola, F., Corleone, G., & Goeman, F. (2022). Dissecting the Epigenome Driving Drug Resistance by ATAC-Seq. In Cancer Drug Resistance (pp. 171-185). Humana, New York, NY.

      3.Kang, R. B., Li, Y., Rosselot, C., Zhang, T., Siddiq, M., Rajbhandari, P., ... & Lu, G. (2023). Single-nucleus RNA sequencing of human pancreatic islets identifies novel gene sets and distinguishes β-cell subpopulations with dynamic transcriptome profiles. Genome Medicine, 15(1), 1-24.

      4.Takeuchi, F., Liang, Y. Q., Shimizu-Furusawa, H., Isono, M., Ang, M. Y., Mori, K., ... & Kato, N. (2023). Gene-regulation modules in nonalcoholic fatty liver disease revealed by single-nucleus ATAC-seq. Life Science Alliance, 6(10).

      5.Tang, X., Lynn, G. E., Cui, Y., Cerny, J., Arora, G., Tomayko, M. M., ... & Fikrig, E. (2023). Bulk and single-nucleus RNA sequencing highlight immune pathways induced in individuals during an Ixodes scapularis tick bite. Infection and Immunity, e00282-23.

      6.Kang, R. B., Lee, J., Varela, M., Li, Y., Rosselot, C., Zhang, T., ... & Lu, G. (2023). Human Pancreatic α-Cell Heterogeneity and Trajectory Inference Analysis Using Integrated Single Cell-and Single Nucleus-RNA Sequencing Platforms. bioRxiv, 2023-11.

      7.Tyshkovskiy, A., Kholdina, D., Ying, K., Davitadze, M., Molière, A., Tongu, Y., ... & Gladyshev, V. N. (2024). Transcriptomic Hallmarks of Mortality Reveal Universal and Specific Mechanisms of Aging, Chronic Disease, and Rejuvenation. bioRxiv, 2024-07.

      8.Yifang Liu, Yuchen Qiu, Keqing Nian and Sara H. Rouhanifard,et al(2024).NuclampFISH enables cell sorting based on nuclear RNA expression for chromatin analysis.bioRxiv.doi: https://doi.org/10.1101/2024.10.30.619948

      9.S. Yamazaki; Utham K. Valekunja; Jing Chen-Roetling; Akhilesh B. Reddy(2024). Heat Shock Factor 1 Governs Sleep-Wake Cycles Across Species.bioRxiv (Cold Spring Harbor Laboratory).DOI 10.1101/2024.11.15.623879




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