作为应用离心管柱滤器提取蛋白及亚细胞结构的先驱

英文特生物技术(北京)有限公司成功研发出五十多个全新的生命科学研究领域蛋白提取产品,其中具有多个特色产品,为蛋白提取分 离带来了新方案, 从原有的溶液法转换为柱式提取法。我公司产品 均具有快捷、易用、产量高及重复性好的特性。在短短几年中,产品 以其稳定的品质获得了世界各国越来越多的科研,药物开发及临床诊 断机构的青睐。



    订购信息:BN-020 / 5243.00元 (20 Preps)

    Minute™ 神经元组织/细胞单个细胞核分离试剂盒 BN-020

    Minute™ Single Nucleus Isolation Kit for Neuronal Tissues/Cells

    描述:

    单细胞RNA测序是研究组织复杂组成的有力技术,然而神经元是高度互联的,很难从神经元组织中,例如脑和脊髓等获得单个细胞。从冷冻的神经元组织中分离出完整的细胞更加困难。由于这些限制,因此单细胞RNA测序可以使用单核测序取代。从神经元组织中分离单个核的传统方法比较繁琐、耗时长,得率低,且很难去除髓鞘和其他细胞成分的污染。本试剂盒专门为解决以上问题而设计,操作简单、快速、且分离效率高。与传统方法相比,该试剂盒所需的起始原料更少,可处理1-25mg范围的样品。缓冲液中含有专用的表面活性剂,可有效的溶解细胞。如需要不含有表面活性剂的完整细胞核分离,可选购cat#NI-024。

    应用:

    FACS分析、单核分析(如RNA-seq和ATAC-seq)、免疫荧光染色、细胞周期分析和凋亡研究等。

    储存条件:

    4℃

    参考文献:BN-020文献列表
    • 1.Klein, K. M., Mascarenhas, R., Merrikh, D., Khanbabaei, M., Maroilley, T., Kaur, N., ... & Tarailo-Graovac, M. (2024). Identification of a novel mosaic MTOR variant in purified neuronal DNA from depth electrodes in a patient with focal cortical dysplasia. medRxiv, 2024-01.

      2.Wojick, J. A., Paranjapye, A., Chiu, J. K., Mahmood, M., Oswell, C., Kimmey, B. A., ... & Corder, G. (2024). A nociceptive amygdala-striatal pathway for chronic pain aversion. bioRxiv, 2024-02.

      3.James, J. G., McCall, N. M., Hsu, A. I., Oswell, C. S., Salimando, G. J., Mahmood, M., ... & Corder, G. (2024). Mimicking opioid analgesia in cortical pain circuits. bioRxiv, 2024-04.

      4.Vornholt, E., Liharska, L. E., Cheng, E., Hashemi, A., Park, Y. J., Ziafat, K., ... & Beckmann, N. D. (2024). Characterizing cell type specific transcriptional differences between the living and postmortem human brain. medRxiv, 2024-05.

      5Guo, X., Keenan, B. T., Reiner, B. C., Lian, J., & Pack, A. I. (2024). Single-nucleus RNA-seq identifies one galanin neuronal subtype in mouse preoptic hypothalamus activated during recovery from sleep deprivation. Cell Reports, 43(5).

      6.Rodriguez de los Santos, M., Kopell, B. H., Buxbaum Grice, A., Ganesh, G., Yang, A., Amini, P., ... & Breen, M. S. (2024). Divergent landscapes of A-to-I editing in postmortem and living human brain. medRxiv, 2024-05.

      7.Kalef-Ezra, E., Friscioni, L., Horner, D., Morley, C., Morrow, G., Guo, Y., & Proukakis, C. (2024). Fluorescence-activated nuclei sorting (FANS) for single-cell Whole Genome Sequencing (scWGS).

      8.Tyshkovskiy, A., Kholdina, D., Ying, K., Davitadze, M., Molière, A., Tongu, Y., ... & Gladyshev, V. N. (2024). Transcriptomic Hallmarks of Mortality Reveal Universal and Specific Mechanisms of Aging, Chronic Disease, and Rejuvenation. bioRxiv, 2024-07.

      9.Mascarenhas, R., Merrikh, D., Khanbabaei, M., Kaur, N., Ghaderi, N., Maroilley, T., ... & Klein, K. M. (2024). Detecting somatic variants in purified brain DNA obtained from surgically implanted depth electrodes in epilepsy. medRxiv, 2024-07.

      10.Rodrigues-Amorim, D., Bozzelli, P. L., Kim, T., Liu, L., Gibson, O., Yang, C. Y., ... & Tsai, L. H. (2024). Multisensory gamma stimulation mitigates the effects of demyelination induced by cuprizone in male mice. Nature Communications, 15(1), 6744.

      11.McSwiggin, H., Wang, R., Magalhaes, R. D. M., Zhu, F., Doherty, T. A., Yan, W., & Jendzjowsky, N. (2024). Comprehensive sequencing of the lung neuroimmune landscape in response to asthmatic induction. bioRxiv, 2024-10.




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