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作为应用离心管柱滤器提取蛋白及亚细胞结构的先驱

英文特生物技术（北京）有限公司成功研发出五十多个全新的生命科学研究领域蛋白提取产品，其中具有多个特色产品，为蛋白提取分 离带来了新方案， 从原有的溶液法转换为柱式提取法。我公司产品 均具有快捷、易用、产量高及重复性好的特性。在短短几年中，产品 以其稳定的品质获得了世界各国越来越多的科研，药物开发及临床诊 断机构的青睐。



    订购信息：LR-042 / 6223.00元(20 Preps)

    Minute™ 质膜脂筏分离试剂盒 LR-042

    Minute™ Plasma Membrane-Derived Lipid Raft Isolation Kit

    描述：

    脂筏是含有高水平胆固醇和鞘脂的小质膜结构域。脂筏被发现存在于质膜（PM）和一些内膜系统中，如线粒体相关膜（MAMS）和内质网等。脂筏参与许多细胞过程，如信号转导，膜转运和蛋白质分选。脂质修饰的蛋白质和一些跨膜蛋白质集中在脂筏中，而其他蛋白质被排除在外。脂筏还被发现与质膜上的Na+ / K+ ATP酶相关。传统的脂筏分离方法是从总膜结构中分离出脂筏，而不能分质膜或者细胞器膜上的脂筏。为了克服传统方法的缺点，我们开发了此款基于离心管柱技术的质膜脂筏分离试剂盒。首先较大的质膜囊泡被分离出来，然后使用含有非离子表面活性剂缓冲液处理，通过台式离心机即可离心分离质膜脂筏部分。不使用传统的匀浆机和超速离心机，1h左右即可高度富集质膜脂筏。


    *如需分离总的脂筏，请使用 Minute™ 总脂筏分离试剂盒 Cat#LR-039


    提示：本试剂盒最终获取的脂筏沉淀，如需进行蛋白实验需使用溶解液溶解后进行下游实验，根据不同下游实验推荐使用WA-009，WA-010，WA-011溶解液。

    应用：

    提取的蛋白可用于SDS-PAGE ，ELISA ，IP，2D胶分析，质谱，酶活检测以及其他应用。

    储存：

    4℃

    参考文献：LR-042文献列表

    • 1.Bu, Y., Teng, Q., Feng, D., Sun, L., Xue, J., & Zhang, G. (2021). YLMY Tyrosine Residue within the Cytoplasmic Tail of Newcastle Disease Virus Fusion Protein Regulates Its Surface Expression to Modulate Viral Budding and Pathogenicity. Microbiology Spectrum, 9(3), e02173-21.

      2.Marissa Rashkovan; Robert Albero; Francesca Gianni; Pablo Perez-Duran; Hannah I Miller; et al.（2021）.Intracellular cholesterol pools regulate oncogenic signaling and epigenetic circuitries in Early T-cell Precursor Acute Lymphoblastic Leukemia.Cancer Discovery.

      3.Shan-Shan Gao; Run Shi; Jing Sun; Yanhong Tang; Jing-Feng Li; et al(2022).GPI Anchored Ligand-BioID2-Tagging System Identifies Galectin-1 Mediating Zika Virus Entry.SSRN Electronic Journal.

      4.Fiore, D., Proto, M. C., Franceschelli, S., Pascale, M., Bifulco, M., & Gazzerro, P. (2022). In Vitro Evidence of Statins’ Protective Role against COVID-19 Hallmarks. Biomedicines, 10(9), 2123.

      5.Lim, J. H., Ahmad, K., Chun, H. J., Hwang, Y. C., Qadri, A. F., Ali, S., ... & Lee, E. J. (2022). IgLON4 Regulates Myogenesis via Promoting Cell Adhesion and Maintaining Myotube Orientation. Cells, 11(20), 3265.

      6.Sha, Y. L., Liu, Y., Yang, J. X., Wang, Y. Y., Gong, B. C., Jin, Y., ... & Zhao, Q. (2022). B3GALT4 remodels the tumor microenvironment through GD2-mediated lipid raft formation and the c-met/AKT/mTOR/IRF-1 axis in neuroblastoma. Journal of Experimental & Clinical Cancer Research, 41(1), 1-21.

      7.Gao, S.-S., Shi, R., Sun, J., Tang, Y., Zheng, Z., Li, J.-F., Li, H., Zhang, J.,Leng, Q., Xu, J., Chen, X., Zhao, J., Sy, M.-S., Feng, L., Li, C., GPI-Anchored Ligand-BioID2-Tagging System Identifies Galectin-1 Mediating Zika Virus Entry, ISCIENCE (2022), doi: https://doi.org/10.1016/j.isci.2022.105481.

      8.Li, B., Ding, Z., Calbay, O. et al. FAP is critical for ovarian cancer cell survival by sustaining NF-κB activation through recruitment of PRKDC in lipid rafts. Cancer Gene Ther (2022). https://doi.org/10.1038/s41417-022-00575-x

      9.Oh, S., Jo, S., Bajzikova, M., Kim, H. S., Dao, T. T., Rohlena, J., ... & Park, S. (2023). Non-bioenergetic roles of mitochondrial GPD2 promote tumor progression. Theranostics, 13(2), 438.

      10.Zhao, Q., Su, H., Jiang, W., Luo, H., Pan, L., Liu, Y., ... & Tan, B. (2023). IGF-1 Combined with OPN Promotes Neuronal Axon Growth in Vitro Through the IGF-1R/Akt/mTOR Signaling Pathway in Lipid Rafts. Neurochemical Research, 1-12.

      11.Mirza, I., Haloul, M., Hassan, C., Masrur, M., Mostafa, A., Bianco, F. M., ... & Mahmoud, A. M. (2023). Adiposomes from Obese-Diabetic Individuals Promote Endothelial Dysfunction and Loss of Surface Caveolae. Cells, 12(20), 2453.

      12.Khalifa, B. A., Guijarro, A., Ravera, S., Bertola, N., Adorni, M. P., Papotti, B., ... & Nencioni, A. (2023). Cyclic fasting bolsters cholesterol biosynthesis inhibitors’ anticancer activity. Nature Communications, 14(1), 6951.

      13.Cheng, M., Zhang, R., Li, J., Ma, W., Li, L., Jiang, N., ... & Wu, Z. (2024). MβCD inhibits SFTSV entry by disrupting lipid raft structure of the host cells. Antiviral Research, 106004.





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